human non small cell lung cancer cell line a549  (ATCC)

Journal: bioRxiv

Article Title: Nanobody MET CAR T cells show efficacy in solid tumors

doi: 10.64898/2026.01.27.702111

Figure Lengend Snippet: (A) mRNA and western blot of MET expression levels in a series of small cell lung cancer cell lines. (B and C) MET expression by flow cytometry and cytotoxicity in NCI-H1341. N= 3 technical replicates in the cytotoxicity assay; P values from two-way ANOVA. (D-E) MET expression by flow cytometry and cytotoxicity in NCI-H146. N= 3 technical replicates; P values calculated using two-way ANOVA. (F) Histogram of MET expression levels in different cell lines. (G) Cytotoxicity of VHH2-CARs against the same cell lines as in (E). N= 3 technical replicates; P values calculated using two-way ANOVA. * P<0.05; **P<0.01; ***P<0.001; ****P<0.0001 .

Article Snippet: Small cell lung cancer cell lines NCI-H146 (ATCC HB173) and NCI-H1341(ATCC CRL-5864) were cultured in DMEM-F12 supplemented with 10% FBS, 1X Insulin-Transferrin-Selenium and 1% penicillin/streptomycin.

Techniques: Western Blot, Expressing, Flow Cytometry, Cytotoxicity Assay

Journal: Journal of Translational Medicine

Article Title: Developing an antibody drug encapsulation nanoagent targeting HER2 for cancer treatment

doi: 10.1186/s12967-025-07450-x

Figure Lengend Snippet: Effects of Tr-ACT2 on a lung cancer cell model. ( A ) Effects of Tr-ACT2 on the growth of A549 cells at concentrations of 0.05 µM, 0.25 µM, and 1 µM for indicated time. Cell growth was evaluated using the MTT assay. ( B ) Representative images of A549 cell morphology at 48 h after treatment with 0.25 μM Tr-ACT2 or vehicle. Scale bar: 400 μm. ( C , D ) Western blot analysis showing the protein levels of PARP, cleaved PARP, IgG heavy chain, IgG light chain, and GAPDH in A549 cells treated with 0.25 μM Tr-ACT2 for indicated time. Protein levels are quantified by densitometry and normalized by GAPDH, versus 0 h. Data are presented as means ± SD ( n ≥ 4). p > 0.05 (ns), p < 0.05 (*), p < 0.01 (**), p < 0.001 (***), p < 0.0001 (****)

Article Snippet: Human breast cancer cell lines SKBR3, JIMT1, BT549, MDA-MB-231, and the human non-small cell lung cancer (NSCLC) cell line A549 were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA), and cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% Fetal bovine serum (FBS) and 1% Antibiotic-Antimycotic, at 37 °C and 5% CO2.

Techniques: MTT Assay, Western Blot

Journal: Journal of Translational Medicine

Article Title: Developing an antibody drug encapsulation nanoagent targeting HER2 for cancer treatment

doi: 10.1186/s12967-025-07450-x

Figure Lengend Snippet: Antitumor efficacy of Tr-ACT2 in the A549 xenograft model. ( A ) Tumor volume change over time for all mice. ( B ) Normalized body weight change over time for all mice. Data are presented as means ± SEM ( n ≥ 3)

Article Snippet: Human breast cancer cell lines SKBR3, JIMT1, BT549, MDA-MB-231, and the human non-small cell lung cancer (NSCLC) cell line A549 were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA), and cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% Fetal bovine serum (FBS) and 1% Antibiotic-Antimycotic, at 37 °C and 5% CO2.

Techniques:

Journal: Discover Oncology

Article Title: Epigenetic remodeling and apoptotic activation by Chidamide suppress small cell lung cancer in molecularly distinct subtypes

doi: 10.1007/s12672-025-04356-4

Figure Lengend Snippet: Dose- and time-dependent suppression of SCLC cell proliferation and clonogenicity by Chidamide. A–C Dose–response curves of H69, H526, and H446 cells treated with Chidamide at various concentrations for 24–96 h, assessed by CCK-8 assay. D Representative images of clonogenic assays 48 h after treatment with Chidamide at IC10, IC20, and IC50 concentrations (Scale bar: 626.1 μm). E–G Quantitative analysis of colony numbers from three independent experiments, and one-way ANOVA followed by Dunnett’s post hoc test was performed (mean ± SD, n = 3 independent experiments; ** P < 0.01, *** P < 0.001)

Article Snippet: Human small cell lung cancer (SCLC) cell lines H69, H526, and H446 were purchased from the American Type Culture Collection (ATCC) and cultured in RPMI-1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS; Gibco), 1% penicillin streptomycin, and 1.5% HEPES at 37 °C, 5% CO 2 , and 90% humidity.

Techniques: CCK-8 Assay

Journal: Discover Oncology

Article Title: Epigenetic remodeling and apoptotic activation by Chidamide suppress small cell lung cancer in molecularly distinct subtypes

doi: 10.1007/s12672-025-04356-4

Figure Lengend Snippet: Dose-dependent apoptosis induction and G1-phase arrest in Chidamide-treated SCLC cells. A Apoptosis analysis by flow cytometry: Representative Annexin V-FITC/PI dot plots (left) and quantified apoptotic rates (histogram, right) of H69, H526, and H446 cells treated with 0.1% DMSO (Control) and Chidamide at IC 10 , IC 20 , and IC 50 concentrations (H69: 0.163, 0.572, 4.9 μM; H526: 0.278, 0.566, 1.979 μM; H446: 0.122, 0.347, 2.073 μM) for 48 h. B Cell cycle analysis: DNA content histograms (left) and quantified G1/S/G2 phase distributions (histogram, right) of cells treated as in A . Data (mean ± SD, n = 3 independent experiments) were analyzed using GraphPad Prism 5 software. Comparisons with the control group were performed using one-way ANOVA and two-way ANOVA followed by Dunnett’s post-hoc test (*** P < 0.001, ns )

Article Snippet: Human small cell lung cancer (SCLC) cell lines H69, H526, and H446 were purchased from the American Type Culture Collection (ATCC) and cultured in RPMI-1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS; Gibco), 1% penicillin streptomycin, and 1.5% HEPES at 37 °C, 5% CO 2 , and 90% humidity.

Techniques: Flow Cytometry, Control, Cell Cycle Assay, Software

Journal: Discover Oncology

Article Title: Epigenetic remodeling and apoptotic activation by Chidamide suppress small cell lung cancer in molecularly distinct subtypes

doi: 10.1007/s12672-025-04356-4

Figure Lengend Snippet: Chidamide alters histone acetylation, cell cycle regulators, and mitochondrial apoptosis in SCLC cells. ( A, D, G ) H69, ( B, E, H ) H526, and ( C, F, I ) H446 cells were treated with Chidamide at indicated concentrations or DMSO control for 48 h. Western blot analysis demonstrated dose-dependent decrease in HDAC1/2/3, increase in Ac-H3 and Ac-H4, downregulation of Cyclin E1 and CDK2, upregulation of p21 and p27, and activation of mitochondrial apoptosis via altered Bcl-2 and Bax expression. GAPDH was used as loading control. Data represent three independent experiments

Article Snippet: Human small cell lung cancer (SCLC) cell lines H69, H526, and H446 were purchased from the American Type Culture Collection (ATCC) and cultured in RPMI-1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS; Gibco), 1% penicillin streptomycin, and 1.5% HEPES at 37 °C, 5% CO 2 , and 90% humidity.

Techniques: Control, Western Blot, Activation Assay, Expressing

Journal: Discover Oncology

Article Title: Epigenetic remodeling and apoptotic activation by Chidamide suppress small cell lung cancer in molecularly distinct subtypes

doi: 10.1007/s12672-025-04356-4

Figure Lengend Snippet: Potent dose-dependent antitumor activity of chidamide with no overt signs of toxicity in SCLC xenografts ( A ) Representative images of subcutaneous tumors derived from H69, H526, and H446 cells in nude mice treated with vehicle (Control), low-dose (12.5 mg/kg), and high-dose (25 mg/kg) Chidamide for 21 days. B–D Tumor volume dynamics in H69, H526, and H446 xenografts, showing significant growth inhibition in Chidamide-treated groups compared to Control. (E–G) Body weight monitoring revealed no significant differences among groups. Data are mean ± SD (n = 3 mice/group); color-coded lines: orange (Control), green (Low Dose), and blue (High Dose). Statistical analysis was performed using GraphPad Prism 5 with two-way ANOVA followed by the Bonferroni test (** P < 0.01, *** P < 0.001, ns )

Article Snippet: Human small cell lung cancer (SCLC) cell lines H69, H526, and H446 were purchased from the American Type Culture Collection (ATCC) and cultured in RPMI-1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS; Gibco), 1% penicillin streptomycin, and 1.5% HEPES at 37 °C, 5% CO 2 , and 90% humidity.

Techniques: Activity Assay, Derivative Assay, Control, Inhibition

Journal: Discover Oncology

Article Title: Epigenetic remodeling and apoptotic activation by Chidamide suppress small cell lung cancer in molecularly distinct subtypes

doi: 10.1007/s12672-025-04356-4

Figure Lengend Snippet: Chidamide promotes apoptosis and necrosis in SCLC xenograft models: H&E and TUNEL analyses. A Representative hematoxylin and eosin (H&E)-stained sections of H69, H526, and H446 xenografts treated with vehicle (Control), low dose (12.5 mg/kg), and high dose (25 mg/kg) Chidamide. Histopathological analysis reveals increased necrotic areas (pink eosinophilic zones) and reduced viable tumor cells in high-dose groups (Scale bar: 60 μm). B–D TUNEL staining (green) combined with DAPI nuclear counterstaining (blue) in H69 ( B ), H526 ( C ), and H446 ( D ) xenografts. Apoptotic cells (TUNEL + /DAPI +) exhibit dose-dependent enrichment, with the highest apoptotic rate in high-dose groups (Scale bar: 50 μm). E–G Quantitative analysis of TUNEL fluorescence intensity in H69 ( E ), H526 ( F ), and H446 ( G ) tumors. Statistical analysis was performed using GraphPad Prism 5 with one-way ANOVA followed by Dunnett’s post-hoc test for comparisons against the control group (mean ± SD, n = 3 biological replicates; * P < 0.05, ** P < 0.01, *** P < 0.001)

Article Snippet: Human small cell lung cancer (SCLC) cell lines H69, H526, and H446 were purchased from the American Type Culture Collection (ATCC) and cultured in RPMI-1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS; Gibco), 1% penicillin streptomycin, and 1.5% HEPES at 37 °C, 5% CO 2 , and 90% humidity.

Techniques: TUNEL Assay, Staining, Control, Fluorescence

Journal: Discover Oncology

Article Title: Epigenetic remodeling and apoptotic activation by Chidamide suppress small cell lung cancer in molecularly distinct subtypes

doi: 10.1007/s12672-025-04356-4

Figure Lengend Snippet: Immunohistochemical and Western blot analyses of histone acetylation, DNA damage markers, and apoptosis-related proteins in Chidamide-treated SCLC xenografts. A–C Immunohistochemical (IHC) staining of formalin-fixed paraffin-embedded tumor sections from H69, H526, and H446 xenografts treated with vehicle (Control), low-dose (12.5 mg/kg), and high-dose (25 mg/kg) Chidamide, assessing Ac-H3, γ-H2AX, p21, and Cleaved caspase-3 expression (Scale bar: 20 μm). D–F Quantification of IHC staining intensity (mean optical density ± SD, n = 3 independent experiments) using Image-Pro Plus software. G Western blot analysis of tumor lysates for H3, Ac-H3, γ-H2AX, p21, Caspase-3, and Cleaved caspase-3. GAPDH served as a loading control. Statistical analysis was performed using GraphPad Prism 5 with two-way ANOVA followed by the Bonferroni test (* P < 0.05, ** P < 0.01, *** P < 0.001, ns )

Article Snippet: Human small cell lung cancer (SCLC) cell lines H69, H526, and H446 were purchased from the American Type Culture Collection (ATCC) and cultured in RPMI-1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS; Gibco), 1% penicillin streptomycin, and 1.5% HEPES at 37 °C, 5% CO 2 , and 90% humidity.

Techniques: Immunohistochemical staining, Western Blot, Immunohistochemistry, Formalin-fixed Paraffin-Embedded, Control, Expressing, Software

Journal: Frontiers in Immunology

Article Title: Study on the spatiotemporal regulation of interferon-stimulated genes during Zika virus infection

doi: 10.3389/fimmu.2025.1702266

Figure Lengend Snippet: Fold change in the expression of the 11 interferon-stimulated genes (ISGs) in A549 cells after Zika virus (ZIKV) infection. Fold change in the expression of the 11 genes in A549 cells was determined 24 h after mock infection or ZIKV infection [multiplicity of infection (MOI) = 1] using RT-PCR. Gene expression was normalized to GAPDH and calculated using the 2 −ΔΔ C t method. Data are presented as the mean ± SD from at least three independent experiments. Statistical significance was assessed using a two-sided **p < 0.01; ***p < 0.001;****p < 0.0001.

Article Snippet: The human non-small cell lung cancer cell line A549 (purchased from the American Type Culture Collection, catalog no. CCL-185) was used.

Techniques: Expressing, Virus, Infection, Reverse Transcription Polymerase Chain Reaction, Gene Expression

Journal: Frontiers in Immunology

Article Title: Study on the spatiotemporal regulation of interferon-stimulated genes during Zika virus infection

doi: 10.3389/fimmu.2025.1702266

Figure Lengend Snippet: Effects of seven interferon-stimulated gene (ISG) knockdown on Zika virus (ZIKV) replication in A549 cells. A549 cells were transfected with small interfering RNA (siRNA) targeting candidate ISGs or non-targeting control siRNA for 48 h, followed by infection with ZIKV at a multiplicity of infection (MOI) of 1. The viral copy number in the supernatant was measured by quantitative real-time PCR (qRT-PCR) at 24 h post-infection. Data represent the mean ± SD from three or more independent experiments. Significance was determined using two-sided Student’s t -test. * p < 0.05; ** p < 0.005.

Article Snippet: The human non-small cell lung cancer cell line A549 (purchased from the American Type Culture Collection, catalog no. CCL-185) was used.

Techniques: Knockdown, Virus, Transfection, Small Interfering RNA, Control, Infection, Real-time Polymerase Chain Reaction, Quantitative RT-PCR